Inhibitory Effect of Probiotics Lactobacillus Supernatants Against Streptococcus Mutans and Preventing Biofilm Formation

Abstract

Probiotic microorganisms can release bioactive substances that can inhibit the growth and biofilm formation of pathogenic microorganisms such as Streptococcus mutans. Dental caries is a multi-factorial chronic infection disease, which starts with bacterial biofilm formation caused mainly by S. mutans. This study investigated the characteristics of Lactobacillus spp. strains as an oral probiotic. Twelve Lactobacillus spp. species obtained from fermented milk and dairy products were identified. Antimicrobial activity was determined with the Minimum Inhibition Concentration against S. mutans as an oral pathogen. Biofilm formation capabilities of the identified Lactobacillus strains in supernatant and culture media were determined. In addition, their ability to α-amylase tolerance and pH values (24h-48h) were determined. L.plantarum showed the highest antimicrobial activity compare other Lactobacillus strains. Also, L. plantarum inhibited biofilm formation.

Keywords

• streptococcus mutans
• probiotic cultures
• biofilm activity
• oral health
• antimicrobial activity