Preparation Process of Alkali-extracted Polysaccharides from Abalone Viscera and Its Repair Activity against H2O2 Oxidative Damage in L929 Cells

Abstract

Objective: The extraction process of alkali-extracted abalone viscera polysaccharides (Aavp) was optimized, and the preventive and repair effects of purified Aavp on H2O2 oxidative damage on L929 cells were studied to provide a reference for the development and application of Aavps. Methods: Crude Aavp was obtained by hot alkali extraction followed by alcohol precipitation, and its extraction process was optimized by the response surface method. The preventive and repair abilities of H2O2 oxidative damage on L929 cells were analyzed. Resulst: The optimum extraction conditions of crude Aavps were as follows: Solid-liquid ratio of 1:150 g/mL, extraction time of 2 h, and extraction temperature of 70 ℃. Under these optimal conditions, the yield of crude Aavp was 8.57%. In the prevention test of H2O2 oxidative damage on L929 cells, different doses of purified Aavp (20, 50 and 100 μg/mL) had a significant preventive effect on L929 cell oxidative damage (P<0.001) but did not show a dose effect. The cell survival rate of the middle dose group (50 μg/mL) was the highest (71.94%±3.08%), which was slightly better than the positive control vitamin E group but not significant. In the repair test, different doses of purified Aavp (20, 50 and 100 μg/mL) had a significant repair effect on the oxidative damage of L929 cells (P<0.001) and showed a dose effect. In contrast, the cell survival rate of the high-dose group (100 μg/mL) was the highest (90.93%±1.17%), slightly higher than that of the positive control vitamin E group (87.96%±3.05%). The results showed that purified Aavp showed an excellent preventive and repair effect on H2O2 oxidative damage in L929 cells. Conclusion: Aavp has an ability to repair H2O2 oxidative damage of L929 cells.

Keywords

• abalone viscera
• alkali-extracted polysaccharide
• response surface optimization
• oxidative damage