Annals of the University Dunarea de Jos of Galati. Fascicle VI: Food Technology (Aug 2013)

Antioxidant and antibacterial properties of capsaicine microemulsions

  • CRISTIAN DIMA,
  • GIGI COMAN,
  • MIHAELA COTÂRLEŢ,
  • PETRU ALEXE,
  • ŞTEFAN DIMA

Journal volume & issue
Vol. 37, no. 1
pp. 39 – 49

Abstract

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The aim of this study was to prepare capsaicin microemulsions and to assess their antioxidant and antibacterial properties. Pseudoternare phase diagrams were made and were highlighted O/W/S/CoS weight ratios corresponding to microemulsion states. The oil phase (O) was soybean oil and for the aqueous phase (W) was used a mixture of water and glycerol in a ratio of 4:1 (wt/wt). As a surfactant (S) was used Tween 40 and cosurfactant (CoS) was ethanol in the mass ratio S:CoS = 2:1. Viscosimetric and conductometric analyses revealed the transition state of the O/W, W/O microemulsions and bicontinuous structures. The antioxidant properties of the capsaicin microemulsions were assayed based on the capacity to counteract DPPH (2,2-diphenyl-1-picrylhydrazyl) radical. The scavenging capacity of the crude capsaicin was IC50 (DPPH) = 2.63±0.34 and for capsaicin microemulsions was IC50 (DPPH) = 5.26±0.28, lower than the value BHT (IC50 = 9.21±0.36μg·mL-1) (p<0.5). Antibacterial activity of capsaicin and capsaicin microemulsions were evaluated using Kirby-Bauer disk diffusion susceptibility tests against three common bacteria: Staphylococcus aureus, Salmonela enterica, and Escherichia coli.

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