Beneficial effects of a selected probiotic mixture administered to high fat-fed mice before and after the development of obesity

Abstract

We investigated the efficacy of different probiotics and bacteria used in dairy products to prevent the development of obesity and ameliorate established obesity in C57BL/6J mice fed a high fat diet (HFD). For the preventive efficacy, Lactobacillus rhamnosus GG, L. acidophilus LA1/K8, a mixture of Bifidobacterium lactis Bi1, B. breve Bbr8 and B. breve BL10 (B. mix), or a mixture of L. bulgaricus Lb2 and S. termophilus Z57 were given to mice before (2 weeks) and together with HFD (12 weeks). B. mix was selected for its anti-obesity activity and assessed in established obesity by feeding mice the HFD for 24 weeks and B. mix for the last 12 weeks. We found that B. mix was able to both prevent and ameliorate established obesity by reducing weight gain, adipose tissue fat accumulation, adipocyte size, and macrophage and CD4+ T cell infiltration, improving lipid profile and regulating leptin and cytokine secretion.

Keywords

• Obesity
• High-fat diet
• Probiotic
• Macrophage
• Adipocyte
• Cytokine