Metabolomics and targeted amino acid analysis reveal the liver protective effect of arginyl-fructosyl-glucose from red ginseng on acute liver injury in mice

Lei Men; Ziyu Wang; Meng Gou; Zhongyu Li; Wei Li; Chunbin Li; Keke Li; Xiaojie Gong

Journal of Functional Foods (Apr 2023)

Metabolomics and targeted amino acid analysis reveal the liver protective effect of arginyl-fructosyl-glucose from red ginseng on acute liver injury in mice

Lei Men,
Ziyu Wang,
Meng Gou,
Zhongyu Li,
Wei Li,
Chunbin Li,
Keke Li,
Xiaojie Gong

Affiliations

Lei Men: Department of Biological Engineering, College of Life Science, Dalian Minzu University, Dalian 116600, China; Key Laboratory of Biotechnology and Bioresources Utilization, Ministry of Education, Dalian Minzu University, Dalian 116600,China
Ziyu Wang: Department of Biological Engineering, College of Life Science, Dalian Minzu University, Dalian 116600, China; Key Laboratory of Biotechnology and Bioresources Utilization, Ministry of Education, Dalian Minzu University, Dalian 116600,China
Meng Gou: Lamprey Research Center, Liaoning Normal University, Dalian 116081, China
Zhongyu Li: Department of Biological Engineering, College of Life Science, Dalian Minzu University, Dalian 116600, China; Key Laboratory of Biotechnology and Bioresources Utilization, Ministry of Education, Dalian Minzu University, Dalian 116600,China
Wei Li: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, Jilin 130118, China
Chunbin Li: Department of Biological Engineering, College of Life Science, Dalian Minzu University, Dalian 116600, China; Key Laboratory of Biotechnology and Bioresources Utilization, Ministry of Education, Dalian Minzu University, Dalian 116600,China
Keke Li: Department of Biological Engineering, College of Life Science, Dalian Minzu University, Dalian 116600, China; Key Laboratory of Biotechnology and Bioresources Utilization, Ministry of Education, Dalian Minzu University, Dalian 116600,China; Corresponding authors at: Department of Biological Engineering, College of Life Science, Dalian Minzu University, Dalian 116600, China.
Xiaojie Gong: Department of Biological Engineering, College of Life Science, Dalian Minzu University, Dalian 116600, China; Key Laboratory of Biotechnology and Bioresources Utilization, Ministry of Education, Dalian Minzu University, Dalian 116600,China; Corresponding authors at: Department of Biological Engineering, College of Life Science, Dalian Minzu University, Dalian 116600, China.

Journal volume & issue: Vol. 103
p. 105473

Abstract

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Arginyl-fructosyl-glucose (AFG) is one of the typical active non-saponins in red ginseng. This study investigates the hepatoprotective effects of AFG against acute liver injury (ALI) induced by D-galactosamine (D-GalN) and lipopolysaccharide (LPS) in mice. A metabolomics study was performed to clarify the hepatoprotective effect mechanisms of AFG on ALI. Eight metabolites were identified in the liver samples as potential biomarkers responsible for the hepatoprotective effect of AFG. The pathway analysis showed that the hepatoprotective effects of AFG were associated with amino acid metabolism and oxidative stress. Amino acid quantitative results revealed that Hyp, Harg, Aad, β-Ala, and Abu significantly changed after the pretreatment with AFG. In addition, AFG pre-treatment attenuated the upregulated Nrf2 and HO-1, and enhanced the expression level of Mrp2 in liver. These results has demonstrated a promising hepatoprotective of AFG for ALI, and AFG is expected to become a functional food with hepatoprotective effect.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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