Araticum (Annona crassiflora) juice is an excellent matrix for Lactobacillus paracasei LBC-81 with high antioxidant potential

Abstract

This study aimed to develop an araticum (Annona crassiflora) juice supplemented with a probiotic strain L. paracasei LBC-81, and evaluate the probiotic strain viability during refrigerated storage period and under in vitro gastrointestinal conditions. Three juices formulations were elaborated containing 20 % (F1), 30 % (F2) and 40 % (F3) of araticum pulp, with and without L. paracasei LBC-81. Changes in the physiochemical properties in the formulations during storage were also evaluated. The viability of L. paracasei in the three formulations during 28 days of storage was significantly greater (P < 0.05) than the recommended minimum (6 log CFU mL−1) for a product to be considered a probiotic. L. paracasei showed significantly (P < 0.05) greater viability in the formulation made with 40 % araticum pulp after 28 days of refrigerated storage. The araticum juices enriched with L. paracasei showed a significantly (P < 0.05) marked decrease and increase in pH and acidity, respectively. The addition of L. paracasei in the araticum juice formulations provided a significant increase (P < 0.05) in the total phenolic content (F1: 1926; F2: 3338.6; F3: 4390.5 and F1:1584; F2: 2629; F3: 3139 μmol galic acid/L juice with and without bacteria, respectively) and greater stability of the antioxidant activity (F1: 1492; F2: 2305; F3: 2618 and F1: 902; F2: 1461; F3: 1450.2 μmol TROLOX/L juice with and without bacteria, respectively). The araticum juice proved to be an excellent matrix for L. paracasei by providing a high percentage of survival during the in vitro gastrointestinal conditions.

Keywords

• Araticum
• Lactobacillus paracasei LBC-81
• Survival
• Antioxidant activity
• In vitro gastrointestinal conditions