Production of Xylooligosaccharide from Cassava Pulp’s Waste by Endo-β-1,4-D-Xylanase and Characterization of Its Prebiotic Effect by Fermentation of <i>Lactobacillus acidophilus</i>
Anak Agung Istri Ratnadewi,
Marena Thalita Rahma,
Nurhayati Nurhayati,
Agung Budi Santoso,
Kartika Senjarini,
Antje Labes,
Muhammad Reza
Affiliations
Anak Agung Istri Ratnadewi
Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember, Jalan Kalimantan 37, Jember 68121, Indonesia
Marena Thalita Rahma
Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember, Jalan Kalimantan 37, Jember 68121, Indonesia
Nurhayati Nurhayati
Graduate School of Biotechnology and PUI-BioTIn, University of Jember, Jember 68121, Indonesia
Agung Budi Santoso
Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember, Jalan Kalimantan 37, Jember 68121, Indonesia
Kartika Senjarini
Department of Biology, Faculty of Mathematics and Natural Sciences, University of Jember, Jalan Kalimantan 37, Jember 68121, Indonesia
Antje Labes
Faculty of Energy und Biotechnology, Flensburg University of Applied Sciences, Kanzleistraße 91-93, 24943 Flensburg, Germany
Muhammad Reza
Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember, Jalan Kalimantan 37, Jember 68121, Indonesia
This study explores the production of prebiotic xylooligosaccharide (XOS) from cassava pulp waste and its effectiveness for the growth of Lactobacillus acidophilus (L. acidophilus). We successfully produced and characterized XOS from cassava pulp xylan using a Bacillus sp. endo-β-1,4-D-xylanase. The XOS was added to modify the MRS medium (MRSm) in various concentrations (0, 1, 3 and 5%) in which the L. acidophilus was inoculated. The growth of L. acidophilus was observed every 12 h for 2 days, and the fermentation products were analyzed for pH, sugar content, and short-chain fatty acids (SCFA) in terms of types and amount. The study showed that L. acidophilus grew well in MRSm. The optimum XOS concentration in MRSm was 5%, indicated by the highest growth of L. acidophilus (8.61 log CFU mL−1). The profile of SCFA products is 14.42 mM acetic acid, 0.25 mM propionic acid, 0.13 mM isobutyric acid, 0.41 mM n-butyric acid, 0.02 mM n-valeric acid, 0.25 mM isovaleric acid, and 25.08 mM lactic acid.
