Stir Bar Sorptive Extraction - New Approach for the Determination of Some Beer Flavours.

Abstract

For the determination of beer flavours well-established methods require sample preparation steps such as liquid/liquid extraction, solid phase extraction or distillation techniques. During the past years the microextraction procedures become popular e. g. solid phase microextraction (SPME) or stir bar sorptive extraction (SBSE). This work presents the simple and rapid possibility of the determination of some beer flavours using stir bar sorptive extraction. A commercially available small stir bar (10 mm length, 1,3 mm OD) is coated with polydimethylsiloxane and produced by Gerstel GmbH and it is known as Twister. The Twister is placed directly in the beer sample and stirred. Then the stir bar is removed, rinsed with distilled water and eluted with 200 μl of organic solvent for gas chromatographic analysis.Validated parameters of the optimised method are shown in this work.For the determination of beer flavours well-established methods require sample preparation steps such as liquid/liquid extraction, solid phase extraction or distillation techniques. During the past years the microextraction procedures become popular e. g. solid phase microextraction (SPME) or stir bar sorptive extraction (SBSE). This work presents the simple and rapid possibility of the determination of some beer flavours using stir bar sorptive extraction. A commercially available small stir bar (10 mm length, 1,3 mm OD) is coated with polydimethylsiloxane and produced by Gerstel GmbH and it is known as Twister. The Twister is placed directly in the beer sample and stirred. Then the stir bar is removed, rinsed with distilled water and eluted with 200 μl of organic solvent for gas chromatographic analysis.Validated parameters of the optimised method are shown in this work.

Keywords

• beer
• beer flavours
• stir bar sorptive extraction (SBSE)