Extractive Fermentation for Recovery of Bacteriocin-Like Inhibitory Substances Derived from <i>Lactococcus lactis</i> Gh1 Using PEG2000/Dextran T500 Aqueous Two-Phase System
Roslina Jawan,
Sahar Abbasiliasi,
Joo Shun Tan,
Murni Halim,
Shuhaimi Mustafa,
Bin Hao Lee,
Jia Sim Kwa,
Arbakariya B. Ariff
Affiliations
Roslina Jawan
Bioprocessing and Biomanufacturing Research Centre, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia
Sahar Abbasiliasi
Halal Products Research Institute, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia
Joo Shun Tan
School of Industrial Technology, Universiti Sains Malaysia, Gelugor 11800, Pulau Pinang, Malaysia
Murni Halim
Bioprocessing and Biomanufacturing Research Centre, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia
Shuhaimi Mustafa
Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia
Bin Hao Lee
PNT Research Sdn. Bhd, Level 36, Menara Maxis, Kuala Lumpur City Centre 50088, Malaysia
Jia Sim Kwa
PNT Research Sdn. Bhd, Level 36, Menara Maxis, Kuala Lumpur City Centre 50088, Malaysia
Arbakariya B. Ariff
Bioprocessing and Biomanufacturing Research Centre, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia
This work aimed to optimize the parameters affecting partitioning of a bacteriocin-like inhibitory substances (BLIS) from Lactococcus lactis Gh1 in extractive fermentation using polyethylene glycol (PEG)/dextran aqueous two-phase system (ATPS). This system was developed for the simultaneous cell cultivation and downstream processing of BLIS. Results showed that the molecular weight of PEG, PEG concentration, and dextran T500 affect the partition coefficient (K), purification factor (PF), and yield of BLIS partitioning. ATPS composed of 10% (w/w) PEG2000 and 8% (w/w) dextran T500, provided the greatest conditions for the extractive BLIS production. The K (1.00 ± 0.16), PF (2.92 ± 0.37) and yield (77.24 ± 2.81%) were increased at selected orbital speed (200 rpm) and pH (pH 7). Sustainable growth of the cells in the bioreactor and repeated fermentation up to the eighth extractive batch were observed during the scale up process, ensuring a continuous production and purification of BLIS. Hence, the simplicity and effectiveness of ATPS in the purification of BLIS were proven in this study.
