Anti-cancer potential of polysaccharide extracted from hawthorn (Crataegus.) on human colon cancer cell line HCT116 via cell cycle arrest and apoptosis

Lei Ma; Guanying Bianca Xu; Xinyu Tang; Cheng Zhang; Wen Zhao; Jie Wang; Hong Chen

Journal of Functional Foods (Jan 2020)

Anti-cancer potential of polysaccharide extracted from hawthorn (Crataegus.) on human colon cancer cell line HCT116 via cell cycle arrest and apoptosis

Lei Ma,
Guanying Bianca Xu,
Xinyu Tang,
Cheng Zhang,
Wen Zhao,
Jie Wang,
Hong Chen

Affiliations

Lei Ma: College of Food Science and Technology, Hebei Agricultural University, Baoding, China
Guanying Bianca Xu: Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana 61801, USA
Xinyu Tang: Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana 61801, USA
Cheng Zhang: Department of Food Science and Engineering, Jinan University, Guangzhou, China
Wen Zhao: College of Food Science and Technology, Hebei Agricultural University, Baoding, China
Jie Wang: College of Food Science and Technology, Hebei Agricultural University, Baoding, China; Corresponding authors at: College of Food Science and Technology, Hebei Agricultural University, Baoding 071001 China (J. Wang). Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, 905 S. Goodwin Ave, Bevier Hall Rm 472, Urbana, IL 61801, USA (H. Chen).
Hong Chen: Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana 61801, USA; Corresponding authors at: College of Food Science and Technology, Hebei Agricultural University, Baoding 071001 China (J. Wang). Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, 905 S. Goodwin Ave, Bevier Hall Rm 472, Urbana, IL 61801, USA (H. Chen).

Journal volume & issue: Vol. 64
p. 103677

Abstract

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This study demonstrated that a homogeneous polysaccharide (HPS) extracted from Hawthorn exerted anti-cancer effects on colon cancer cells. Human colon cancer cell line HCT116 were treated with 125–1000 µg/mL HPS for 12 h and subsequent analysis was performed on proliferation and signaling pathways. HPS significantly inhibited proliferation of HCT116 cells when analyzed using WST-1 assay and immunofluorescence staining of Ki-67. Flow cytometric studies revealed that HPS could arrest the cell cycle in the S and G2/M phases and increase the rate of apoptosis. HPS downregulated the expression of Cyclin A1/D1/E1 and CDK-1/2. HPS induced apoptosis on HCT116 cells through upregulation of caspase3, 7, 8, 9 and Fas, FADD, TNF-R1, TRADD, upregulation of caspase3 protein was also observed. HPS has an anti-cancer potential in the treatment of human colon cancer which makes it a potential candidate in functional foods for cancer patients.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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