Structural characterization and antitumor effects of chitosan oligosaccharides against orthotopic liver tumor via NF-κB signaling pathway

Pan Zou; Shoujun Yuan; Xin Yang; Yongze Guo; Linna Li; Chengwang Xu; Xingchen Zhai; Jing Wang

Journal of Functional Foods (Jun 2019)

Structural characterization and antitumor effects of chitosan oligosaccharides against orthotopic liver tumor via NF-κB signaling pathway

Pan Zou,
Shoujun Yuan,
Xin Yang,
Yongze Guo,
Linna Li,
Chengwang Xu,
Xingchen Zhai,
Jing Wang

Affiliations

Pan Zou: Department of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, 92 West Dazhi Street, Nangang District, Harbin 150090, China; Key Laboratory of Agro-product Quality and Safety, Institute of Quality Standard & Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Haidian District, Beijing 100081, China; Department of Pharmacology and Toxicology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Haidian District, Beijing 100850, China; Tianjin Institute of Agricultural Quality Standard and Testing Technology, Tianjin Academy of Agricultural Sciences, Xiqing District, Tianjin 300381, China
Shoujun Yuan: Department of Pharmacology and Toxicology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Haidian District, Beijing 100850, China
Xin Yang: Department of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, 92 West Dazhi Street, Nangang District, Harbin 150090, China; Key Laboratory of Agro-product Quality and Safety, Institute of Quality Standard & Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Haidian District, Beijing 100081, China; Corresponding authors at: Department of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, 92 West Dazhi Street, Nangang District, Harbin 150090, China (X. Yang). Key Laboratory of Agro-product Quality and Safety, Institute of Quality Standard & Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Haidian District, Beijing 100081, China (J. Wang).
Yongze Guo: Tianjin Institute of Agricultural Quality Standard and Testing Technology, Tianjin Academy of Agricultural Sciences, Xiqing District, Tianjin 300381, China
Linna Li: Department of Pharmacology and Toxicology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Haidian District, Beijing 100850, China
Chengwang Xu: Department of Pharmacology and Toxicology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Haidian District, Beijing 100850, China
Xingchen Zhai: Department of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, 92 West Dazhi Street, Nangang District, Harbin 150090, China; Department of Pharmacology and Toxicology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Haidian District, Beijing 100850, China
Jing Wang: Department of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, 92 West Dazhi Street, Nangang District, Harbin 150090, China; Key Laboratory of Agro-product Quality and Safety, Institute of Quality Standard & Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Haidian District, Beijing 100081, China; Corresponding authors at: Department of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, 92 West Dazhi Street, Nangang District, Harbin 150090, China (X. Yang). Key Laboratory of Agro-product Quality and Safety, Institute of Quality Standard & Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Haidian District, Beijing 100081, China (J. Wang).

Journal volume & issue: Vol. 57
pp. 157 – 165

Abstract

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Increasing studies have reported the antitumor effects of chitosan oligosaccharides (COS) against multiple cancer, including S180, colon tumor, etc. Here, the structural characters of COS were identified and its inhibitory effects against orthotopic liver tumor and immunoregulation effects were also studied. Degree of polymerization (DP) of COS is 2–6, degree of deacetylation (DD) is 98.38 ± 1.34%. COS is positive charged with zeta potential at 11.27 ± 1.56 mV, with diameter at about 100 nm. COS is protective to S180-bearing mouse, activates macrophage Ana-1, and promotes M1 polarization. The antitumor effects of COS against LM3 and HepG2 orthotopic liver tumor in vivo were measured combined with bioimaging system. The mRNA differential analysis of LM3 cell and HepG2 hallmarks alterations indicated that COS inhibits hepatoma via NF-κB pathway, which regulates PI3K/Akt, p38 MAPK, p53 and mitochondria-apoptotic signaling pathways. This study suggests that COS can be developed into an excellent antitumor candidate for liver tumor.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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