مجله پژوهشهای علوم و صنایع غذایی ایران (Jul 2023)
Investigating the Quality and Safety Characteristics of Skin Collagen of Bighead (Hypophthalmichthys nobilis), Silver Carp (Hypophthalmichthys molitrix), Grass Carp (Ctenopharyngodon idella), and Common Carp (Cyprinus carpio) Cultured
Abstract
Collagen has diverse general and biomedical applications and its important role in the future of society have made it a key biopolymer for human health and well-being. Therefore, the present study was conducted with the objectives of extracting collagen from the skin of farmed carp, determining the quality characteristics of collagen, and comparing them. Collagen was extracted from the skin of carp fishes by acidic enzymatic method using 0.5 M acetic acid and pepsin in 48 hours. Collagen treatments (5 treatments) included collagen prepared from the skin of common species, grass carp, bighead, silver, and cow (control). Collagen treatments were not capable of hemolysis and did not show toxic effects on human fibroblast cells. Heavy metals (0.01-0.18 ppm) in collagen extracted from cultured carp species were within the standard range. The color (brightness) of experimental collagen (92.74-93.68) and control (92.38) showed no significant difference (p<0.05). Amino acids cysteine and tryptophan were not observed in collagen. Glycine and hydroxylysine amino acids (352 and 3 residues 1000g-1, respectively) had the highest and lowest amounts in collagen. Amino acids profile and collagen production efficiency (10.51-10.59%) did not show significant differences in carp fish species (p<0.05). Based on the results of the present study, production efficiency, safety and quality characteristics of collagen in cultured carp species did not show any significant difference (p<0.05), and no significant difference was observed between these characteristics and the control (p<0.05). Therefore, the skin of these species can be used to produce collagen and introduce it to the industry as a substitute for mammalian collagen.
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