Non-digestible galactomannan oligosaccharides from Cassia seed gum modulate microbiota composition and metabolites of human fecal inoculum

Miao Miao; Yuqin Shi; Yanxiao Li; Zhengqiang Jiang; Jun Liu; Shaoqing Yang

Journal of Functional Foods (Nov 2021)

Non-digestible galactomannan oligosaccharides from Cassia seed gum modulate microbiota composition and metabolites of human fecal inoculum

Miao Miao,
Yuqin Shi,
Yanxiao Li,
Zhengqiang Jiang,
Jun Liu,
Shaoqing Yang

Affiliations

Miao Miao: Key Laboratory of Food Bioengineering (China National Light Industry), College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua Donglu, Haidian District, Beijing 100083, China
Yuqin Shi: Key Laboratory of Food Bioengineering (China National Light Industry), College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua Donglu, Haidian District, Beijing 100083, China
Yanxiao Li: Bioresource Utilization Laboratory, College of Engineering, China Agricultural University, No. 17 Qinghua Donglu, Haidian District, Beijing 100083, China
Zhengqiang Jiang: Key Laboratory of Food Bioengineering (China National Light Industry), College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua Donglu, Haidian District, Beijing 100083, China
Jun Liu: Key Laboratory of Food Bioengineering (China National Light Industry), College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua Donglu, Haidian District, Beijing 100083, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China; Corresponding authors at: Key Laboratory of Food Bioengineering (China National Light Industry), College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua Donglu, Haidian District, Beijing 100083, China.
Shaoqing Yang: Key Laboratory of Food Bioengineering (China National Light Industry), College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua Donglu, Haidian District, Beijing 100083, China; Corresponding authors at: Key Laboratory of Food Bioengineering (China National Light Industry), College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua Donglu, Haidian District, Beijing 100083, China.

Journal volume & issue: Vol. 86
p. 104705

Abstract

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The digestibility and prebiotic potential of galactomannan oligosaccharides prepared from Cassia seed gum (CMOS) was studied. CMOS were tolerant against simulated gastrointestinal digestion. During in vitro human fecal fermentation with CMOS, the microbiota composition was significantly changed with promoted growth of potential beneficial genera (i.e. Bifidobacterium, Lactobacillus, Bacteroides, Veillonella) while that of potential harmful genera (i.e. Fusobacterium, Lachnospiraceae, and Sutterella) was inhibited. Short chain fatty acids were produced with decreased medium pH, while acetic acid (20.85 mM) and propionic acid (19.77 mM) were the predominant metabolites. For CMOS utilization, α-galactosidase and β-mannosidase were involved with the enzyme activity reached the maximum after 3 h fermentation. The HPAEC chromatograms showed that CMOS with DP = 3 and 4 were prior utilized through enzymatic hydrolysis within 36 h fermentation. Findings gained here will introduce the utilization and targeted production of CMOS (DP = 3 and 4) as prebiotics with defined structural features.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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