Anti-adipogenic effect of erucin in early stage of adipogenesis by regulating Ras activity in 3T3-L1 preadipocytes

Seung Young Chae; Sang Gwon Seo; Hee Yang; Jae Gak Yu; Su Jin Suk; Eun Sun Jung; Hae Ji; Jung Yeon Kwon; Hyong Joo Lee; Ki Won Lee

Journal of Functional Foods (Dec 2015)

Anti-adipogenic effect of erucin in early stage of adipogenesis by regulating Ras activity in 3T3-L1 preadipocytes

Seung Young Chae,
Sang Gwon Seo,
Hee Yang,
Jae Gak Yu,
Su Jin Suk,
Eun Sun Jung,
Hae Ji,
Jung Yeon Kwon,
Hyong Joo Lee,
Ki Won Lee

Affiliations

Seung Young Chae: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea
Sang Gwon Seo: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea; WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea; Advanced Institutes of Convergence Technology, Seoul National University, Suwon, Republic of Korea
Hee Yang: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea; WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea
Jae Gak Yu: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea; WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea
Su Jin Suk: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea; Interdisciplinary Program in Agricultural Biotechnology Major, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea
Eun Sun Jung: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea; WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea
Hae Ji: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea; WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea
Jung Yeon Kwon: Advanced Institutes of Convergence Technology, Seoul National University, Suwon, Republic of Korea
Hyong Joo Lee: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea; WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea; Advanced Institutes of Convergence Technology, Seoul National University, Suwon, Republic of Korea
Ki Won Lee: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea; WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea; Advanced Institutes of Convergence Technology, Seoul National University, Suwon, Republic of Korea; Interdisciplinary Program in Agricultural Biotechnology Major, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea; Corresponding author. Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea. Tel.: +82 2 880 4662; fax: +82 2 873 5095.

Journal volume & issue: Vol. 19
pp. 700 – 709

Abstract

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Erucin is a natural isothiocyanate found in rocket salad and broccoli which are believed to have health benefits including weight control effect and used as a functional food globally. Though anti-cancer effect of erucin has been intensively studied, anti-obesity effect of erucin and its molecular mechanisms have not yet been reported. In this study, we investigated anti-adipogenic effect of erucin in 3T3-L1 preadipocytes and its underlying molecular mechanisms. Erucin inhibited adipogenesis of 3T3-L1 preadipocytes and reduced adipogenic gene expressions. This anti-adipogenic activity of erucin was most effective at early stage of adipogenesis including mitotic clonal expansion (MCE). Fluorescence-activated cell sorter (FACS) analysis and trypan blue assay results showed that erucin induced G1/S arrest and inhibited cell proliferation at MCE stage. Western blot analysis results showed that erucin inhibited extracellular signal-regulated kinase (ERK) signaling pathway by blocking Ras activity. Taken together, these results indicated that erucin inhibits adipogenesis of 3T3-L1 preadipocytes by blocking Ras activity at early stage of adipogenesis.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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