MICROBIAL GENERATION OF SILVER NANOPARTICLES BY SYNTHESIZING ENTEROCOCCUS FAECALIS STRAIN MI103

Abstract

AgNPs have recently attracted attention for their potential as a source of new antimicrobials, with the potential to better fight a wide range of pathogenic bacteria. The use of nanotechnology and nanomaterials has been incorporated into common applications and objects found in our daily lives. A new treatment for antibiotic-resistant bacteria has been proposed using silver nanoparticles. The samples were randomly pooled from tropical soil and water for synthesis of AgNPs. The synthesizing organism, Enterococcus faecalis strain MI103 was identified using 16S rRNA gene sequencing method. The selected bacteria isolate synthesized AgNPs by reduction of AgNO3 solution (incubated for 2 days at room temperature). Colour development from pale yellow to brown was observed for as indication of the extracellular production of AgNPs. AgNPs was further characterized by UV/VIS spectroscopy, Scanning Electron Microscopy (JOEL-JSM-7660F) (shape) and Energy Dispersive X-ray (EDX) (composition). The synthesized AgNPs were of size range of 90-100 nm and were of cylindrical shape. It was found that the highest quantity of AgNO3 was produced at pH 7. Due to its wide use, AgNPs strongly stand the chances of being best nanoparticle in the nearest future.

Keywords

• lagos lagoon
• coliforms
• salinity
• pollution
• physiochemical parameters
• escherichia coli