PIGMENT CONTENTS OF Sargassum polycistum MACROALGAE LIPID FROM SAYANG HEULANG BEACH, INDONESIA

Abstract

Macroalgae has a rich source of potential bioactive compounds. This study aims to identify the pigment profiles of Sargassum polycistum lipid collected from Sayang Heulang Beach, West Java, Indonesia. Lipid was isolated from Sargassum polycistum using chloroform: methanol (2:1, v/v), based on Folch method, which is the initial part of pigment extraction process. Pigment extract was identified by using Thin Layer Chromatography (TLC) with hexane: acetone (7:3; v/v) as eluent. The results showed six spots which successfully identified. Moreover, the spots were separated by using coloumn chromatography and followed characterization by UV-Vis spectroscopy in range 400-900 nm. The experimental results revealed that Sargassum polycistum lipid contained four pigments namely phaeophytin a, α-carotene, β-carotene and fucoxanthin.

Keywords

• alpha-carotene
• beta-carotene
• fucoxanthin
• phaeophytin-a
• Sargassum polycistum