Screening and Identification of Aflatoxin B1 Degradation Strains and Preliminary Exploration of Effective Degradation Components

Abstract

Aflatoxin B1 (AFB1) is a type of fungaltoxin with strong toxicity and strong carcinogenicity. To screen degrading bacteria of AFB1, co-culture of fungi strains which were stored in laboratory and AFB1 was carried out and the strains with the highest degradation rate were chosen. Species of the chosen strains were determined through morphology and ITS rDNA analysis. After separation of different components (bacterial suspension, thalli, spore suspension and fermentation liquor) in strains, the effective components for AFB1 degradation were explored, and the effective components thalli was broken for further investigation. Results showed that the AFB1 degrading rates by Penicillium herquei MD1 bacterial suspension cultured by 72 h and the unbroken thalli were 98.15% and 28.20%, respectively. The degradation of AFB1 was better after the fragmentation of the thalli, and the degradation rate of AFB1 was 55.40% after 24 h of incubation. Therefore, the active components in MD1 bacterial suspension and thalli can degrade AFB1 effectively. The screened MD1 expands the degrading bacterial library of AFB1 and provide some references to biological degradation of AFB1.

Keywords

• aflatoxin b1
• penicillium herquei
• biodegradation
• bioactive ingredients
• identification