Characterization of sea buckthorn polysaccharides and the analysis of its regulatory effect on the gut microbiota imbalance induced by cefixime in mice

Xinyao Xie; Yongcheng Song; Xiufang Bi; Xiaocui Liu; Yage Xing; Zhenming Che

Journal of Functional Foods (May 2023)

Characterization of sea buckthorn polysaccharides and the analysis of its regulatory effect on the gut microbiota imbalance induced by cefixime in mice

Xinyao Xie,
Yongcheng Song,
Xiufang Bi,
Xiaocui Liu,
Yage Xing,
Zhenming Che

Affiliations

Xinyao Xie: Sichuan Key Laboratory of Food Biotechnology, School of Food and Bioengineering, Xihua University, Chengdu 610039, China
Yongcheng Song: Sichuan Key Laboratory of Food Biotechnology, School of Food and Bioengineering, Xihua University, Chengdu 610039, China
Xiufang Bi: College of Food Science and Technology, Southwest Minzu University, Chengdu 610041, China; Corresponding author at: College of Food Science and Technology, Southwest Minzu University, Chengdu 610041, China.
Xiaocui Liu: Sichuan Key Laboratory of Food Biotechnology, School of Food and Bioengineering, Xihua University, Chengdu 610039, China; Key Laboratory of Food Non-thermal Processing, Engineering Technology Research Center of Non-thermal Food Processing, Yibin Xihua University Research Institute, Yibin 644004, China
Yage Xing: Sichuan Key Laboratory of Food Biotechnology, School of Food and Bioengineering, Xihua University, Chengdu 610039, China
Zhenming Che: Sichuan Key Laboratory of Food Biotechnology, School of Food and Bioengineering, Xihua University, Chengdu 610039, China

Journal volume & issue: Vol. 104
p. 105511

Abstract

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Sea buckthorn pomace is a by-product of sea buckthorn products that is not effectively utilized. This study obtained sea buckthorn polysaccharides (SPs) from pomace, analyzed its structure, and investigated its regulatory effect on the gut microbiota imbalance induced by cefixime. The results showed that SPs had an average molecular weight of 6.26 × 103 kDa and mainly consisted of galacturonic acid, galactose, and rhamnose. Biochemical analysis showed that SPs increased the concentration of short-chain fatty acids (SCFAs) and the abundance of Proteobacteria, Verrucomicrobia, and Akkermanis in the gut of cefixime-treated mice. Correlation analysis suggested that various microorganisms had a significant (p < 0.05) relationship with SCFAs. Consequently, the underlying mechanism of SPs in restoring cefixime-induced gut microbiota disorder may be due to the promotion of SCFAs and SCFA-producing bacteria in the gut and gut microbiota regulation, benefiting gut health. This study is significant for developing and utilizing sea buckthorn pomace.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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