Effects of One-Step Abrupt Temperature Change on Anaerobic Co-Digestion of Kitchen Waste with Dewatered Sludge

Abstract

The operating temperature of anaerobic digesters should be adjusted to adapt to seasonal variations in environmental temperature and the composition of organic solid waste. This study investigated the effects of one-step abrupt temperature changes (from mesophilic to thermophilic temperature, M–T, and from thermophilic to mesophilic temperature, T–M) and the inoculation ratio on methane yield and microbial diversity during the anaerobic co-digestion of kitchen waste with dewatered sludge. The results showed that the cumulative methane yield (CMY) level resulting from thermophilic control and the M–T digesters was greater than that resulting from mesophilic control and the T–M digesters. The CMF of M–T digesters increased, whereas the CMY of T–M digesters gradually decreased with an increase in the inoculation ratio. The maximal CMY was 385.1 mL/g-VSSadded, which corresponded to an M–T digester with a 5% inoculation ratio. In the later stage of anaerobic digestion, the bacterial community of T–M was more diverse than that of M–T, but the archaeal community of M–T was more diverse than that of T–M. The one-step temperature change from thermophilic to mesophilic temperature was more stable than that from mesophilic to thermophilic temperature.

Keywords

• ammonia
• inoculum ration
• methane yield
• bacteria
• archaea