Shipin Kexue (Oct 2023)

Simultaneous Determination of Vitamins A, D and E in Foods for Special Medical Purposes by Online Solid Phase Extraction Combined with Two-Dimensional Liquid Chromatography

  • ZHAI Hongwen, MA Hongyu, MA Junmei, CAO Meirong, WANG Jing, WANG Juan, LI Qiang

DOI
https://doi.org/10.7506/spkx1002-6630-20220923-241
Journal volume & issue
Vol. 44, no. 20
pp. 315 – 320

Abstract

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A rapid method for the determination of VA, VD (VD2, VD3) and VE (α-, β-, γ-, and δ-vitamin E) in foods for special medical purposes was established using online solid phase extraction (SPE) combined with two-dimensional liquid chromatography (2D-LC). After saponification, enrichment and purification of VA, VD and VE from samples were accomplished on an online SPE cartridge. VA and four VEs were quantitatively analyzed by the first-dimensional LC, and VD was transferred by heart-cutting to the second-dimensional chromatographic system for separation and detection. SPE columns with different fillings, sample loading solvents, and eluent composition were evaluated, and 2D-LC conditions were systematically optimized. Good linearity was achieved for all vitamins tested with correlation coefficients (R2) greater than 0.997. The mean recoveries of the developed method were 84.4%–109.0% with relative standard deviation (RSD) of 0.34%–6.55% (n = 6). This method was highly automated with low detection errors and high detection efficiency, and could be used for the analysis of vitamins in foods for special medical purposes.

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