Bioassay-guided purification of Lippia citriodora polyphenols with AMPK modulatory activity

María de la Luz Cádiz-Gurrea; Mariló Olivares-Vicente; María Herranz-López; David Arraez-Roman; Salvador Fernández-Arroyo; Vicente Micol; Antonio Segura-Carretero

Journal of Functional Foods (Jul 2018)

Bioassay-guided purification of Lippia citriodora polyphenols with AMPK modulatory activity

María de la Luz Cádiz-Gurrea,
Mariló Olivares-Vicente,
María Herranz-López,
David Arraez-Roman,
Salvador Fernández-Arroyo,
Vicente Micol,
Antonio Segura-Carretero

Affiliations

María de la Luz Cádiz-Gurrea: Department of Analytical Chemistry, University of Granada, Avda. Fuentenueva s/n, 18071 Granada, Spain; Research and Development of Functional Food Centre (CIDAF), PTS Granada, Avda. Del Conocimiento s/n., Edificio BioRegion, 18016 Granada, Spain
Mariló Olivares-Vicente: Instituto de Biología Molecular y Celular (IBMC), Universidad Miguel Hernández, Elche, Alicante, Spain
María Herranz-López: Instituto de Biología Molecular y Celular (IBMC), Universidad Miguel Hernández, Elche, Alicante, Spain
David Arraez-Roman: Department of Analytical Chemistry, University of Granada, Avda. Fuentenueva s/n, 18071 Granada, Spain; Research and Development of Functional Food Centre (CIDAF), PTS Granada, Avda. Del Conocimiento s/n., Edificio BioRegion, 18016 Granada, Spain; Corresponding author at: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda. Fuentenueva s/n, Granada 18071, Spain.
Salvador Fernández-Arroyo: Biomedical Research Unit, Medicine and Surgery Department, Rovira i Virgili University, 43201 Reus (Tarragona), Spain
Vicente Micol: Instituto de Biología Molecular y Celular (IBMC), Universidad Miguel Hernández, Elche, Alicante, Spain; CIBER: CB12/03/30038, Fisiopatología de la Obesidad y la Nutrición, CIBERobn, Instituto de Salud Carlos III (ISCIII), Palma de Mallorca, Spain
Antonio Segura-Carretero: Department of Analytical Chemistry, University of Granada, Avda. Fuentenueva s/n, 18071 Granada, Spain; Research and Development of Functional Food Centre (CIDAF), PTS Granada, Avda. Del Conocimiento s/n., Edificio BioRegion, 18016 Granada, Spain

Journal volume & issue: Vol. 46
pp. 514 – 520

Abstract

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Recently, the relationship between nutrients and health is becoming since natural dietary products as polyphenols are being considered by their potential for the management of several diseases. We aimed to investigate the capacity of Lippia citriodora compounds to modulate AMP-activated protein kinase activity (AMPK) on a hypertrophic adipocyte model. HPLC semi-preparative purification method and reverse phase high performance liquid chromatography coupled to time-of-flight mass detection with electrospray ionization (RP-HPLC-ESI-TOF/MS) were used to obtain de compounds from L. citriodora extract. AMPK activity was measured on the hypertrophic 3T3-L1 adipocyte model by immunofluorescence microscopy. Four compounds of 29 total compounds have been tentatively identified in L. citriodora for the first time by HPLC-ESI-TOF-MS. Phenylpropanoids (verbascoside), iridoids (gardoside) and flavonoids (luteolin-7-diglucoronide) were the best candidates to account for activating AMPK capacity. The combination of specific polyphenols from L. citriodora, which showed strong activating AMPK capacity, could be an alternative in the management of obesity-associated diseases.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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