Investigating in vitro antiobesity properties of enzyme-assisted hydrolysate and its crude sulfated polysaccharide from Padina arborescens

Abstract

In this study, we aimed to evaluate the chemical characteristics and potential antiobesity effects of celluclast-assisted hydrolysate from Padina arborescens (PAC) and its sulfated polysaccharide from Padina arborescens (SPPA) examined in differentiated 3 T3-L1 cells. Brown seaweed derived sulfated polysaccharide is known to possess functional ingredients, which show vivid biological potentials. PAC and SPPA treatment significantly inhibited the intracellular lipid accumulation. Furthermore, SPPA significantly regulated key adipogenic peroxisome proliferator-activated receptor γ (PPAR-γ), CCAAT/enhancer-binding protein α (C/EBP-α), lipogenic peroxisome proliferator-activated receptor γ (PPAR-γ), CCAAT/enhancer-binding protein α (C/EBP-α) and lipolytic adipose triglyceride lipase (ATGL), and AMP-activated protein kinase (AMPK) in 3 T3-L1 cells. In addition, Alamara Blue staining results indicated that energy expenditure was considerably enhanced by SPPA in zebrafish embryos. Thus, our research findings indicate that the PAC and SPPA treatment exerted potential intracellular lipid inhibitory activity and may prove useful as raw materials for food or for the functional food industry.

Keywords

• Intracellular lipid inhibitory activity
• Padina arborescens
• Sulfated polysaccharide
• Adipogenesis
• Lipogenesis
• 3T3-L1