Cytological evaluation by Caco-2 and KU812 of non-allergenic peptides from simulated digestion of infant formula in vitro

Zihao Xu; Hao Bai; Xin Ma; Yong Wu; Zhihua Wu; Anshu Yang; Weixiang Mao; Xin Li; Hongbing Chen

Food Science and Human Wellness (May 2023)

Cytological evaluation by Caco-2 and KU812 of non-allergenic peptides from simulated digestion of infant formula in vitro

Zihao Xu,
Hao Bai,
Xin Ma,
Yong Wu,
Zhihua Wu,
Anshu Yang,
Weixiang Mao,
Xin Li,
Hongbing Chen

Affiliations

Zihao Xu: State Key Laboratory Food Science and Technology, Nanchang University, Nanchang 330047, China; School of food science and Technology, Nanchang University, Nanchang 330047, China
Hao Bai: State Key Laboratory Food Science and Technology, Nanchang University, Nanchang 330047, China; School of food science and Technology, Nanchang University, Nanchang 330047, China
Xin Ma: State Key Laboratory Food Science and Technology, Nanchang University, Nanchang 330047, China; School of food science and Technology, Nanchang University, Nanchang 330047, China
Yong Wu: State Key Laboratory Food Science and Technology, Nanchang University, Nanchang 330047, China; Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China; Jiangxi Province Key Laboratory of Food Allergy, Nanchang University, Nanchang 330047, China
Zhihua Wu: State Key Laboratory Food Science and Technology, Nanchang University, Nanchang 330047, China; Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China; Jiangxi Province Key Laboratory of Food Allergy, Nanchang University, Nanchang 330047, China
Anshu Yang: State Key Laboratory Food Science and Technology, Nanchang University, Nanchang 330047, China; Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China; Jiangxi Province Key Laboratory of Food Allergy, Nanchang University, Nanchang 330047, China
Weixiang Mao: Jiangxi Institute of Quality and Standardization, Nanchang 330046, China; Corresponding authors.
Xin Li: State Key Laboratory Food Science and Technology, Nanchang University, Nanchang 330047, China; School of food science and Technology, Nanchang University, Nanchang 330047, China; Jiangxi Province Key Laboratory of Food Allergy, Nanchang University, Nanchang 330047, China; Corresponding authors.
Hongbing Chen: State Key Laboratory Food Science and Technology, Nanchang University, Nanchang 330047, China; Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China; Jiangxi Province Key Laboratory of Food Allergy, Nanchang University, Nanchang 330047, China

Journal volume & issue: Vol. 12, no. 3
pp. 817 – 824

Abstract

Read online

Milk allergy is a common allergic reaction found in infants and young children, most of them appear tolerance after growing up. In this study, infant formula was digested by simulated in vitro digestion method. The potential non-allergenic peptides were further screened from undigested products by exclusion of the known epitopes from β-lactoglobulin (BLG) and α-lactalbumin (ALA). These potential non-allergenic peptides were synthesized and their transferability were determined by Caco-2 cell monolayer model. Finally, the potential allergenicity were evaluated by KU812 cell degranulation model. The results showed that 7 peptides were screened as non-allergenic sequences, among which were 3 from ALA and 4 from BLG. The Caco-2 cell model showed that all the synthetic peptides were absorbed and transported well. However, only peptide BLG107-118 showed potential allegencity by KU812 model. In conclusion, six peptides, including ALA29–51, ALA80–90, ALA94–103, BLG1–20, BLG24–50, and BLG123–139 were evaluated as hypoallergenic peptides, which could be used for candidates of peptides inducing immune tolerance for persons with milk allergy.

Published in Food Science and Human Wellness

ISSN: 2213-4530 (Online)
Publisher: Tsinghua University Press
Country of publisher: China
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.sciopen.com/journal/2097-0765

About the journal

Abstract

Keywords