nvestigation of antibacterial effects of cell suspension culture and comparison by essential oils and seed extract in Bunium persicum

Abstract

Recently by no incident there is a great concern in people to consume herbal antibiotics rather than using chemical ones due to bacterial resistances witnessed in chemical treatments. In this research the antibacterial activity of aqueous and ethanol extracts of black zira cell suspension culture in comparison with seed extracts and essential oils were investigated against Staphylococus aurous, Escherichia coli and Bacillus subtilis using disk diffusion and digging hole methods. The bacterial concentration was at 106 and 107 CFU/mL. Positive and negative controls were antibiotics (Gentamicin [10 µg/disk], Tetracycline [30 µg/disk]) and solvents (5% DMSO and 80% Ethanol) respectively. Inhibitory effects were studied by measuring the growth inhibiting circle diameter. The Results showed that the range of Minimum Inhibitory Concentration (MIC) were 3.12-6.25, 12.5-50 and 25-50 mg/ml, for the essential oil, the cell extract and the seed extract respectively. According to the results a promising antibacterial activities were revealed from all black zira samples against tested bacteria with the maximum activity to the S. aurous. In contrast to E. coli, the effect of extracts on S. aurous and B. subtilis was dependent to the bacteria concentration. Overall antibacterial effects of cell extracts against tested bacteria were lesser than essential oils (100%) and higher than seed extracts and different dilution of essential oils, therefore it seems cell suspension culture of black zira is a suitable method to produce antibacterial compounds.

Keywords

• antibacterial effect
• bunium persicum
• cell suspension culture
• essential oils
• extract