A RAPID METHOD OF DETERMINATION OF TRANS-RESVERATROL IN ROMANIAN COMMERCIAL WINES BY REVERSED-PHASE HPLC

Abstract

Resveratrol (trans-3,5,4’-trihydroxystilbene) is a phytoalexin produced naturally by 72 different plant species especially grapevines, Japanese knotweed and pines in response to some exogenous factors such as UV radioation, chemical stress and fungal parasites. With the discovery of the „French paradox” resveratrol had entered in the spotlight of researchers. The French paradox is a term generated in 1992 that refers to the cardioprotective effects of resveratrol that have been discovered based on data from French people that has shown an inverse correlation between wine consumption and the incidence of coronary heart disease (CHD) despite the consumption of a diet in hight saturated fat. The aim of the present study is to determine the trans-resveratrol content in 11 Romanian comercial wine samples by direct injection into the HPLC instrument coupled with diode array detector after being filtered through 0.45 µm PTFE membrane filtres. The total resveratrol content in alayses samples varied from 0.03 mg/L to 6.64 g/L. Pinot Noir wines had the highest average resveratrol content, while the lowest was found for the Sauvignion Blanc variety. All wines analyzed contained resveratrol in variable quantities. This study could contribute to the analysis made on the variety of Romanian wines for resveratrol content.

Keywords

• trans-resveratrol
• phytoalexin
• wines
• hplc
• „french paradox”