Ultrasonic extraction, composition analysis, in vitro antioxidant and antiproliferative activities of Mango kernel oil from Jinhuang Mango kernel

Abstract

Mango kernel oil (MKO) contains a large number of symmetric triglycerides, such as stearic acid, oleic acid and palmitic acid. If MKO is used effectively, it can reduce the waste of resources and environmental pollution. In this study, ultrasonic extraction, composition analysis, in vitro antioxidant and antiproliferative activities of Jinhuang mango kernel oil (JMKO) from Jinhuang mango kernel (JMK) as a product were studied for the first time. The ultrasound extraction yield of JMKO was 15.32%. Forty-three compounds and their relative contents were identified by GC-MS. The total relative content was about 95.71%. JMKO showed moderate antioxidant activities in ABTS assay (0.08 mg/mL as IC50 value), DPPH test (IC50 value = 0.27 mg/mL). Furthermore, the reducing power of JMKO was dose dependent, and the reducing capacity of the oil was inferior to vitamin C (Vc), which is known to be a strong reducing agent. When concentrations of Vc and JMKO were 11.6 μg/mL and 0.29 mg/mL, their WA (the total anti-oxidation activity) values were 2.3 and 0.502, respectively. The IC50 of JMKO against TE-1 cancer cells, HeLa cancer cells, and MCF-7 cancer cells were (0.43 ± 0.03) mg/mL, (0.83 ± 0.04) mg/mL, and (0.27 ± 0.02) mg/mL, respectively. The results showed JMKO could be employed as natural antioxidants and health care products

Keywords

• Jinhuang Mango kernel oil (JMKO)
• Chemical constituents
• GC-MS
• Antioxidant activity
• Antiproliferative activity