Lactococcus lactis JCM5805 activates anti-viral immunity and reduces symptoms of common cold and influenza in healthy adults in a randomized controlled trial

Takeo Shibata; Masaya Kanayama; Munetaka Haida; Shuhei Fujimoto; Takashige Oroguchi; Kenji Sata; Nobutaka Mita; Tomoko Kutsuzawa; Mayumi Ikeuchi; Mayu Kondo; Kimitoshi Naito; Michio Tsuda; Yasuhiro Nishizaki; Naoaki Ishii

Journal of Functional Foods (Jun 2016)

Lactococcus lactis JCM5805 activates anti-viral immunity and reduces symptoms of common cold and influenza in healthy adults in a randomized controlled trial

Takeo Shibata,
Masaya Kanayama,
Munetaka Haida,
Shuhei Fujimoto,
Takashige Oroguchi,
Kenji Sata,
Nobutaka Mita,
Tomoko Kutsuzawa,
Mayumi Ikeuchi,
Mayu Kondo,
Kimitoshi Naito,
Michio Tsuda,
Yasuhiro Nishizaki,
Naoaki Ishii

Affiliations

Takeo Shibata: Department of Molecular Life Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, Japan; Life Care Center, Graduate School of Medicine, Tokai University, 143 Shimokasuya, Isehara, Kanagawa, Japan
Masaya Kanayama: Central Laboratories for Key Technologies, Kirin Company, Limited, 1-13-5 Fukuura, Kanazawa-ku, Yokohama, Kanagawa, Japan
Munetaka Haida: Tokai University Junior College of Nursing and Medical Technology, 4-1-1 kitakaname, Hiratsuka, Kanagawa, Japan
Shuhei Fujimoto: Department of Bacteriology and Bacterial Infection, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, Japan
Takashige Oroguchi: Department of Molecular Life Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, Japan; Life Care Center, Graduate School of Medicine, Tokai University, 143 Shimokasuya, Isehara, Kanagawa, Japan
Kenji Sata: Life Care Center, Graduate School of Medicine, Tokai University, 143 Shimokasuya, Isehara, Kanagawa, Japan; Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, Japan
Nobutaka Mita: Life Care Center, Graduate School of Medicine, Tokai University, 143 Shimokasuya, Isehara, Kanagawa, Japan; Department of Physical Recreation, School of Physical Education, Tokai University, 4-1-1 kitakaname, Hiratsuka, Kanagawa, Japan
Tomoko Kutsuzawa: Department of Nursing, School of Health Sciences, Tokai University, 4-1-1 kitakaname, Hiratsuka, Kanagawa, Japan
Mayumi Ikeuchi: Life Care Center, Graduate School of Medicine, Tokai University, 143 Shimokasuya, Isehara, Kanagawa, Japan; Department of Nursing, School of Health Sciences, Tokai University, 4-1-1 kitakaname, Hiratsuka, Kanagawa, Japan
Mayu Kondo: Department of Arts, School of Humanities and Culture, Tokai University, 4-1-1 kitakaname, Hiratsuka, Kanagawa, Japan
Kimitoshi Naito: Life Care Center, Graduate School of Medicine, Tokai University, 143 Shimokasuya, Isehara, Kanagawa, Japan; Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, Japan
Michio Tsuda: Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, Japan
Yasuhiro Nishizaki: Life Care Center, Graduate School of Medicine, Tokai University, 143 Shimokasuya, Isehara, Kanagawa, Japan; Health Screening Center, Tokai University Tokyo Hospital, 1-2-5 Yoyogi, Shibuya-ku, Tokyo, Japan; Digestive and Liver Disease Center, Tokai University Tokyo Hospital, 1-2-5 Yoyogi, Shibuya-ku, Tokyo, Japan
Naoaki Ishii: Department of Molecular Life Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, Japan; Life Care Center, Graduate School of Medicine, Tokai University, 143 Shimokasuya, Isehara, Kanagawa, Japan; Corresponding author. Department of Molecular Life Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan. Tel.: +81 463 93 1121 (ext. 2651); fax: +81 463 94 8884.

Journal volume & issue: Vol. 24
pp. 492 – 500

Abstract

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The effects of heat-killed Lactococcus lactis JCM5805 (JCM5805) on the pathogenesis of common cold and influenza were evaluated. A randomized, placebo-controlled, double-blinded study was conducted and 396 healthy subjects (placebo group n = 205, JCM5805 group n = 191) completed the intervention. Subjects consumed placebo capsules or JCM5805 capsules containing approximately 100 billion cells of heat-killed JCM5805 per day for 12 wk. Significantly fewer incident days were observed in the JCM5805 group than in the placebo group for the symptoms of sore throat (p < 0.05) and cough (p < 0.001). Cell surface expression of HLA-DR on plasmacytoid dendritic cells (pDC) and the expression of VIPERIN in peripheral blood mononuclear cells (PBMCs) were higher post-intervention compared to baseline in the JCM5805 group only (p < 0.05). The expression of IFNA in PBMCs was significantly higher in the JCM5805 group (p < 0.05). These data suggest that the intake of heat-killed JCM5805 stimulated pDC-related anti-viral immunity to mitigate the incidence of common cold and influenza symptoms.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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