Phosvitin and its hydrolysate promote differentiation and inhibit TNF-ɑ induced inflammation in MC3T3-E1 cells via ERK and AKT pathways

Abstract

Phosvitin and its hydrolysate were reported to show antioxidant, anti-inflammation and antimicrobial activities. Given the critical role of inflammation on bone health, the work was designed to study the effects of phosvitin and its hydrolysate on osteoblast differentiation and inflammation in osteoblastic (MC3T3-E1) cells. Both phosvitin and its hydrolysate promoted osteoblast differentiation by upregulating expression of runt-related transcription factor 2, alkaline phosphatase (by phosvitin hydrolysate), osteocalcin production (by phosvitin) and collagen synthesis. They also inhibited TNF-ɑ induced inflammation by reducing the production of regulated on activation, normal T cell expressed and secreted and monocyte chemoattractant protein-1, which are crucial for recruiting osteoclast progenitor and maintaining osteoclast functions, and cyclooxygenase-2, a proinflammatory protein. Adding phosvitin and its hydrolysate activated both extracellular signal–regulated kinases (ERK) and protein kinase B (AKT) signaling pathways, indicating that both phosvitin and its hydrolysate promote osteoblast differentiation and exert antiinflammation effects via ERK and AKT pathways.

Keywords

• Phosvitin
• MC3T3-E1
• Differentiation
• RUNX2
• Inflammation
• Signaling pathway