Prevention of tumor progression in inflammation-related carcinogenesis by anti-inflammatory and anti-mutagenic effects brought about by ingesting fermented brown rice and rice bran with Aspergillus oryzae (FBRA)

Abstract

Fermented brown rice and rice bran with Aspergillus oryzae (FBRA) has shown chemopreventive effectiveness against inflammation-related carcinogenesis, by inhibiting inflammation. We found that Aspergillus oryzae is the main component of the anti-inflammatory effect of FBRA and that the intestinal flora is involved. Mice fed FBRA were found to have a lower tumor-forming rate than mice fed the basic diet. Tumor cell lines obtained from basic diet-fed mice metastasized to distant organs. By contrast, those from FBRA-fed mice acquired reduced metastatic capacity. Micronucleus testing detected somatic mutations in tumor cell lines directly established from primary tumors; these decreased in a dose-dependent manner in FBRA-fed mice. To determine whether FBRA ingestion induced systemic anti-mutagenicity, micronucleus testing was performed using reticulocytes in mice injected with typical mutagens. Mutations induced by these mutagens were suppressed in FBRA-fed mice. These results indicate that anti-inflammation and anti-mutagenicity induced by FBRA intake suppressed the inflammation-related carcinogenic process.

Keywords

• Inflammation-related carcinogenesis
• Chemoprevention
• Tumor progression
• Metastasis
• Somatic mutation
• Fermented brown rice and rice bran with Aspergillus oryzae (FBRA)