Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology (Nov 2017)

Inhibitory Potential Of Lactobacillus Plantarum on Escherichia Coli

  • Laura MITREA,
  • Lavinia Florina CĂLINOIU,
  • Gabriela PRECUP,
  • Maria BINDEA,
  • Bogdan RUSU,
  • Monica TRIF,
  • Lorand Jozsef FERENCZI,
  • Bianca Eugenia ŞTEFĂNESCU,
  • Dan Cristian VODNAR

DOI
https://doi.org/10.15835/buasvmcn-fst:0031
Journal volume & issue
Vol. 74, no. 2
pp. 99 – 101

Abstract

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An alternative therapy to diarrhoeal episodes induced by E. coli is represented by probiotic strains (e.g Lactobacillus), which are able to maintain the gut micro-flora in optimal parameters. This work highlights the inhibitory potential of probiotics (L. plantarum ATCC 8014) over the enteropathogenic bacteria like E. coli ATCC 25922. For this study we co-incubated L. plantarum with E. coli in MRS broth at different concentrations (109, 108 CFU/mL – L. plantarum with 105, 104 CFU/mL – E. coli, 4 groups) for 24, 48 and 72h at 37oC. After co-incubation, E. coli colonies developed on Levine media were counted towards the control. E. coli colonies decreased after 48 and 72h of co-incubation with L. plantarum. Also, was noticed that L. plantarum (108 CFU/mL) completely inhibits E. coli (104 CFU/mL) after 72h of co-incubation. This work proves the inhibitory potential of probiotic strains (L. plantarum ATCC 8014) against enteropathogenic bacteria (E. coli ATCC 25922).

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