Protective effect of Caffeic Acid Phenethyl Ester (CAPE) against oxidative stress

Ana Laura Carreño; Efrain Alday; Jael Quintero; Lucía Pérez; Dora Valencia; Ramón Robles-Zepeda; Judith Valdez-Ortega; Javier Hernandez; Carlos Velazquez

Journal of Functional Foods (Feb 2017)

Protective effect of Caffeic Acid Phenethyl Ester (CAPE) against oxidative stress

Ana Laura Carreño,
Efrain Alday,
Jael Quintero,
Lucía Pérez,
Dora Valencia,
Ramón Robles-Zepeda,
Judith Valdez-Ortega,
Javier Hernandez,
Carlos Velazquez

Affiliations

Ana Laura Carreño: Department of Chemistry-Biology, University of Sonora, Blvd. Luis Encinas y Rosales s/n, Hermosillo, Sonora C.P. 83000, Mexico
Efrain Alday: Department of Chemistry-Biology, University of Sonora, Blvd. Luis Encinas y Rosales s/n, Hermosillo, Sonora C.P. 83000, Mexico
Jael Quintero: Departamento de Ciencias de la Salud, Universidad de Sonora, Blvd. Bordo Nuevo s/n, Ejido Providencia, Cd Obregón, Sonora C.P. 85039, Mexico
Lucía Pérez: Departamento de Investigación en Ciencia y Tecnología, DICTUS, University of Sonora, Blvd. Luis Encinas y Rosales s/n, Hermosillo, Sonora C.P. 83000, Mexico
Dora Valencia: Department of Chemical Biological and Agropecuary Sciences, University of Sonora, Av. Universidad and Irigoyen, Caborca, Sonora C.P. 83600, Mexico
Ramón Robles-Zepeda: Department of Chemistry-Biology, University of Sonora, Blvd. Luis Encinas y Rosales s/n, Hermosillo, Sonora C.P. 83000, Mexico
Judith Valdez-Ortega: Department of Chemistry-Biology, University of Sonora, Blvd. Luis Encinas y Rosales s/n, Hermosillo, Sonora C.P. 83000, Mexico
Javier Hernandez: Unidad de Servicios de Apoyo en Resolución Analítica, Universidad Veracruzana, Xalapa, Ver. C.P. 575, Mexico
Carlos Velazquez: Department of Chemistry-Biology, University of Sonora, Blvd. Luis Encinas y Rosales s/n, Hermosillo, Sonora C.P. 83000, Mexico; Corresponding author.

Journal volume & issue: Vol. 29
pp. 178 – 184

Abstract

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The antioxidant properties of several polyphenolics of propolis have been reported, however their protective effect against oxidative stress considering cell integrity is scarce. In this study, we evaluated the cellular antioxidant activity (CAA) of caffeic acid phenetyl ester (CAPE), rutin and galangin (Sonoran propolis constituents) using two murine cell lines derived from different immunological lineages (B-cell lymphoma and macrophages), based on the fluorescence of intracellularly oxidised 2′-7′-dichlorofluorescein (DCF) probe, together with cell morphology analysis and membrane integrity assessment by flow cytometry. CAPE (5 μM) showed the highest CAA (97.9%) on B-cell lymphoma cells against 1 mM H2O2, followed by rutin (25 μM; 30.9%), meanwhile galangin (25 μM) did not show CAA. CAPE exhibited a higher CAA than the antioxidant controls [quercetin (12.5 μM), ascorbic acid (50 μM) and trolox (10 μM)], and additionally it helped to preserve cellular morphology. Similar effects were observed on macrophage cells, indicating that CAPE has a cellular protective effect against ROS. Further studies are needed to investigate the potential health benefits of CAPE.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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