Effect of Temperatures during the Lye Macerating on the Structure of Tripe Collagen

Abstract

In this paper, acidase compound method was used to extract collagen from tripe. The effects of NaOH rising temperature (40~60 ℃) on collagen structure of tritrium hairy were analyzed by UV spectrophotometer, fluorescence spectrophotometer, infrared spectrometer and scanning electron microscope. The results showed that the the lye macerating of tripe could decrease the UV absorption intensity of collagen in tribolus. With the increase of temperature during the lye macerating of tripe, the fluorescence intensity of collagen decreased and the content of sulfhydryl group increased. It indicated that heating treatment will gradually deepen the degeneration degree of collagen and eventually complete degeneration. When the temperature was 60 ℃, the surface hydrophobicity of tribolus collagen decreased. With the increase of temperature during the lye macerating of tripe, the microstructure of collagen of tripe showed that collagen molecules shrunk, gradually curled, accelerate agglutination, gap decrease and flake collagen was broken. Therefore, NaOH can destroy the secondary structure of collagen in tripe, presenting as fracture, fragmentation, stretching and loosening, and different temperature of hyperplasia has a significant impact on the structure of collagen.

Keywords

• tripe
• collagen
• temperature
• protein structure characterization