Lactiplantibacillus plantarum L67 reduces diet-induced obesity by stimulating gene programming for adipose lipolysis and energy expenditure

Abstract

Obesity and its associated metabolic diseases, including diabetes and fatty liver diseases, represent a significant global health concern. While substantial evidence highlights the role of probiotic bacteria in alleviating diet-induced obesity, the effect of probiotics on lipolysis in relation to diet-induced obesity remains unexplored. This study demonstrated that administration of Lactiplantibacillus plantarum L67 (L67) ameliorated diet-induced obesity, lipid accumulation in adipose and liver tissues, and insulin resistance and improved cold tolerance in high-fat diet (HFD)-fed mice by regulating the expression of lipolysis- and energy dissipation-related genes. Notably, L67 administration increased PPARγ deacetylation by increasing Sirt1 expression, which, in turn, regulated the expression of genes related to lipolysis (ATGL and G0S2) and thermogenesis (UCP1 and PGC-1α), facilitating lipolysis, and enhancing mitochondrial levels and oxygen consumption rates in adipocytes. Overall, these findings suggest that L67 protects against diet-induced obesity and associated metabolic abnormalities by stimulating lipolysis and energy expenditure.

Keywords

• Lactiplantibacillus plantarum L67
• Obesity
• Adipose lipolysis
• Energy homeostasis
• Insulin resistance
• Insulin sensitivity