DETERMINATION OF PRIMERS EFFICIENCY IN THE DETECTION OF PEDIOCOCCUS IN WINES

Abstract

Pediococcus spp. are lactic acid bacteria which are considered spoilage in the wine making processes. Early detection can minimize the negative effects of Pediococcus spp on wine quality and prevent the development of advanced spoilage stages. The goal of this work was development and testing of a fast and efficient Real-Time Polymerase Chain Reaction (R-T PCR)-based method for easy detection of Pediococcus spp. strains in wine at the early stages of wine spoilage. As a result, primer sets for the Real time-PCR were designed to allow for correct and efficient detection of Pediococcus spp., and experimentally tested.

Keywords

• dilutions
• dna
• lactic bacterium
• microbiological
• realtime pcr
• wines spoilage