DETERMINATION OF CATECHOLAMINES AND RELATED MOLECULES IN BRAIN EXTRACT USING A HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY MASS SPECTROMETRY METHOD

Abstract

The present paper presents a hydrophilic interaction chromatography (HILIC) coupled to tandem mass spectrometry method for the analysis of 11 catecholamines, precursors and metabolites in a sheep brain extract. The LODs were between 0.5 ng∙mL-1 (3-MT) and 250 ng∙mL-1 (DOPAC). The method accuracy has been evaluated in brain extract by recovery studies with samples spiked at a concentration of 100 ng∙mL-1. For the spiked brain extract, the mean recovery values ranged from 81 % to 101 %, with precision < 8 %.

Keywords

• hilic
• neurotransmitters
• sheep brain extract
• tandem mass spectrometry
• tsk gel amide 80.