Arginine supplementation reduces colonic injury, inflammation and oxidative stress of DSS-induced colitis in mice

Abstract

This study evaluated the mechanisms action of arginine in experimental model of ulcerative colitis. C57BL/6 mice were randomized into 4 groups: Control group (C): standard diet; Colitis group (Col): standard diet and DSS solution; Pretreated group (PT): diet supplementation with arginine before and during colitis induction; Treated group (T): diet supplementation with arginine during colitis induction. Colitis was induced by administration of 1.5% DSS for 5 days. Parameters such as intestinal permeability (IP), bacterial translocation (BT), histological and morphometric analysis, cytokines, immunoglobulin A, inflammatory infiltrate, oxidative stress, tight junction, iNOS1 Nitric oxide indutase.1 were performed. The Col group showed increased IP, BT, oxidative stress and inflammatory infiltrate in the colon. Arginine decreased IP, BT, oxidative stress and inflammatory infiltrate in the colon. Only, the group T reduced iNOS, cytokine IL-17,2 Interleukine 17.2 and increased collagen area and cytokine TGF-β.3 Transforming growth factor beta.3 These results suggest that arginine can constitute in potential therapy for colitis.

Keywords

• Arginine
• DSS-induced colitis
• Intestinal mucosa
• Collagen
• Nitric oxide