Shipin gongye ke-ji (Feb 2024)

Research Progress of Glucansucrase from Lactic Acid Bacteria

  • He SHI,
  • Liansheng YU,
  • Xintong QI,
  • Zhigang QIAN,
  • Lianbao KAN,
  • Renpeng DU

DOI
https://doi.org/10.13386/j.issn1002-0306.2023040042
Journal volume & issue
Vol. 45, no. 4
pp. 354 – 361

Abstract

Read online

Glucansucrase (EC.2.4.5.1) is a class of α-glucosyltransferases, mainly produced by lactic acid bacteria such as Leuconostoc, Streptococcus and Lactobacillus. The structure and catalytic mechanism of glucansucrase are diverse, and it is an important tool enzyme for the biosynthesis of exopolysaccharide. This article mainly reviews the source, classification, structure and reaction mechanism of glucansucrase and the effects of medium composition, culture conditions on the production of glucansucrase, with a focus on the optimization methods, separation and purification processes, and enzymatic properties of glucansucrase, and prospects for its development trend. This article aims to provide reference for the research of glucansucrase in related fields.

Keywords