Quality evaluation of the extract of aerial parts from Atractylodes lancea based on fingerprint and chemometrics

Abstract

The high-performance liquid chromatography (HPLC) fingerprint technique is an effective method to evaluate the quality, identify species, ensure consistency and stability of traditional Chinese medicine (TCM). In this study, determination was performed on an Agilent ZORBAX SB-C18 (4.6 × 250 mm, 5 µm) column with methanol-water as mobile phase in gradient elution at the flow rate of 0.8 mL/min, the column temperature was 30°C, the detection wavelength was set to 260 nm. The HPLC fingerprints of 10 batches of extract of the aerial parts from Atractylodes lancea were evaluated by similarity analysis and chemometrics including hierarchical cluster analysis, principal component analysis. Meanwhile, the antibacterial activities of 10 batches of extract of the aerial parts from Atractylodes lancea were estimated on Escherichia coli (E. coli). The correlation coefficient of similarity between 10 batches of extract was ≥ 0.998, and the extract of the aerial parts from Atractylodes lancea have stable effects on inhibiting the proliferation of E. coli. Besides, bivariate correlation analysis (BCA) and partial least squares regression (PLSR) were applied to construct a spectrum-effect relationship, which proved the 5 main marker compounds were more relevant to anti-bacteria. Therefore, in this work, the results were successfully built an agile and efficient method that can ensure the intra-assay stability of biological activity of the extract of the aerial parts from Atractylodes lancea.

Keywords

• Atractylodes lancea
• Antibacterial activity
• Chemometrics
• Similarity analysis
• High-performance liquid chromatography