Effect of paraprobiotic prepared from Kimchi-derived Lactobacillus plantarum K8 on skin moisturizing activity in human keratinocyte

Abstract

Paraprobiotics, which are inactivated microbial cells or cell fractions that can confer beneficial effects upon uses, have garnered attention. In this study, we investigated the paraprobiotic effect of Kimchi-derived Lactobacillus plantarum K8 on skin moisturizing activity in human keratinocyte. Four species of lactic acid bacteria were prepared into cell lysates under high pressure. L. plantarum K8 lysate induced outstanding hyaluronic acid production in HaCaT cells, compared with the lysates of L. lactis, L. sakei, and Streptococcus thermophilus. L. plantarum K8 lysate prepared under high pressure of 27,000 psi with three passage times was optimized for inducing hyaluronan production. Results of real-time PCR and western blotting analyses indicated that L. plantarum K8 lysate could enhance skin-moisturizing activity via upregulating hyaluronic acid synthase-2 and aquaporin-3 expression in HaCaT cells. Collectively, our results present preliminary data for utilizing L. plantarum K8 paraprobiotic as a functional ingredient in moisturizing products.

Keywords

• Lactobacillus plantarum
• High pressurization
• Moisturizing
• Hyaluronic acid
• Hyaluronan synthase
• Aquaporin