Optimization of the Enzymatic Acylation Reaction Conditions of Vitis davidii Malvidin

Abstract

In order to increase the structural stability of anthocyanins, taking Vitis davidii malvidin as sample and the lipase CAL-B as catalyst, the effects of reaction time, temperature, and substrate molar ratio on enzymatic acylation were investigated. Based on the single factor experiments and taking the acylation rate as the response value, a response surface optimization experiment was designed to obtain the optimal process parameters for the enzymatic acylation reaction of Vitis davidii malvidin. Results showed that, the optimized parameters were as follows: The reaction time was 43 h, reaction temperature was 54 °C, substrate molar ratio was 1:24. Under these conditions, the acylation rate of malvidin was 49.92%±0.12%, this paper would provide important ideas for the stabilization and industrial production of Vitis davidii malvidin.

Keywords

• vitis davidii
• malvidin
• enzymatic acylation
• reaction condition