Dihydromyricetin protects against cerebral ischemia/reperfusion injury via suppressing microglia-mediated neuroinflammation and activation of ERK1/2-CREB signaling pathway

Yafei Zhao; Panpan Wang; Shuangshuang Chen; Chaojun Han; Qiuting Yan; Longtai Zheng; Jia Jia; Zhaoxiang Ren; Xuechu Zhen

Journal of Functional Foods (Jun 2017)

Dihydromyricetin protects against cerebral ischemia/reperfusion injury via suppressing microglia-mediated neuroinflammation and activation of ERK1/2-CREB signaling pathway

Yafei Zhao,
Panpan Wang,
Shuangshuang Chen,
Chaojun Han,
Qiuting Yan,
Longtai Zheng,
Jia Jia,
Zhaoxiang Ren,
Xuechu Zhen

Affiliations

Yafei Zhao: Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases and College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215021, China; The Collaborative Innovation Center for Brain Science, Soochow University, Suzhou, China
Panpan Wang: Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases and College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215021, China; The Collaborative Innovation Center for Brain Science, Soochow University, Suzhou, China
Shuangshuang Chen: Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases and College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215021, China; The Collaborative Innovation Center for Brain Science, Soochow University, Suzhou, China
Chaojun Han: Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases and College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215021, China; The Collaborative Innovation Center for Brain Science, Soochow University, Suzhou, China
Qiuting Yan: Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases and College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215021, China; The Collaborative Innovation Center for Brain Science, Soochow University, Suzhou, China
Longtai Zheng: Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases and College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215021, China; The Collaborative Innovation Center for Brain Science, Soochow University, Suzhou, China
Jia Jia: Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases and College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215021, China; The Collaborative Innovation Center for Brain Science, Soochow University, Suzhou, China
Zhaoxiang Ren: Corresponding authors at: Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215021, China.; Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases and College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215021, China; The Collaborative Innovation Center for Brain Science, Soochow University, Suzhou, China
Xuechu Zhen: Corresponding authors at: Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215021, China.; Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases and College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215021, China; The Collaborative Innovation Center for Brain Science, Soochow University, Suzhou, China

Journal volume & issue: Vol. 33
pp. 76 – 84

Abstract

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Dihydromyricetin (DHM), a natural flavonoid compound extracted from the fruit Ampelopsis grossedentata, exerts various pharmacological effects. We explored the neuroprotective effects of DHM following cerebral ischemia. Male ICR mice were intraperitoneally injected with DHM for 7 days. Post-ischemic neurological deficits were evaluated with behavioral tests. Mice brain tissues were harvested for infarction analysis, immunohistochemistry. The production of pro-inflammatory mediators in microglial cells were assessed by qPCR and ELISA. Neuroprotective effects of DHM were assessed in HT22 neuronal cells subjected to oxygen-glucose deprivation (OGD)/reoxygenation. DHM reduced the activation of microglia, protected HT22 neurons against OGD-induced injury and promoted functional recovery following middle cerebral artery occlusion (MCAO). Mechanistically, DHM reduced release of pro-inflammatory mediators from microglia. In addition, DHM suppressed caspase-3 cleavage and upregulated CREB, Bcl-2 and phosphorylation of ERK1/2 in HT22 cells. Our study suggested that DHM protects against cerebral ischemia by suppressing microglial neuroinflammation and activating neuronal ERK-CREB-Bcl-2 signaling pathway.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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