Prevalence of genes encoding the classical enterotoxins of Staphylococcus aureus isolated from buffalo milk in Tabriz area by multipex PCR

Abstract

Isolation, identification and grouping of staphylococcal enterotoxins in milk is of great importance, since these toxins are considered as a major potential source of humans illness. In this study, 75 staphylococcus aureus strains isolated from buffalo milk were assessed. For this, extracted DNA was analysed by multiplex-PCR for the presence and diversity of genes encoding the common classical staphylococcal enterotoxins. Results revealed that one of the isolates haboured both sec and seb genes; meanwhile, three other isolates contained merely sec gene. Moreover, the gene encoding the production of enterotoxin-A was not detected in any of the isolates. It was concluded that the diversity of genes encoding the classical enterotoxins of S. aureus isolated from Tabriz area was low.

Keywords

• Staphylococcus aureus
• Enterotoxins
• Buffaloe milk
• Multiplex-PCR