Biodegradation of Some Pesticides by Enterobacter cloacae; in vitro

Abstract

The degradation of some pesticides (acetamiprid, boscalid, deltamethrin, kresoxim-methyl, pyraclostrobin and pyridaben) by Enterobacter cloacae (plant growth-promoting bacteria) was investigated in vitro. In this study, firstly the effective substances used initially did not have a negative effect on E. cloacae, and the bacteria showed the same growth as the control group in the nutrient agar medium. Secondly, application doses of pesticides were added to erlenmeyer with nutrient broth, and 1 ml of 1×109 cells/ml of E. cloacae solution was inoculated and incubated in a shaker at room temperature (24°C) for 7 days. After the nutrient broth medium was extracted, pesticides were measured by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). When the results were compared with the control group, there was no degradation in acetamiprid, but 98.9% in pyraclostrobin, 98.6% in boscalid, 94.1% in kresoxim-methyl, 73.5% in pyridaben and 57.3% in deltamethrin. It is thought that the results of this study and the information obtained on the degradation of boscalid, deltamethrin, kresoxim-methyl, pyraclostrobin and pyridaben will shed light on future studies.

Keywords

• biyodegredasyon
• enterobacter cloacae
• pestisit kalıntısı
• pgpr
• lc-ms/ms