Identification of Proteins and Determination of Metal Contents in Two Kinds of Macrocybe lobayensis

Abstract

To deeply explore and develop their nutritive values and functions, the proteins and metal elements in two kinds of Macrocybe lobayensis (ML), TG505 and Grand Canyon ML were determined and analyzed. Protein fingerprints were obtained using phenol extraction and two-dimensional electrophoresis (2DE), and highly expressed proteins were selected. The enzymatic hydrolysates of highly expressed proteins were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Functional analysis of these proteins was carried out using bioinformatics tools. The contents of metal elements in the two kinds of mushrooms were determined by inductively coupled plasma mass spectrometry (ICP-MS). The results showed that 33 highly expressed proteins were identified from each kind of ML, which were involved in the growth, development, genetics, stress resistance, stress response, pharmaceutical activity and other functions of ML. For both kinds of ML, the major metal elements were K, Mg, Mo, Ca, Al, and Fe in decreasing order. The present study not only provides guidance for further exploitation and utilization of ML, with both medicinal and culinary uses, but also is of reference significance for the research and development of tropical edible mushrooms in Hainan.

Keywords

• macrocybe lobayensis; protein fingerprint; functional analysis; metal elements