Research Progress in the Separation of Bioactive Peptides Using Mixed-Mode Chromatography

Abstract

Bioactive peptides are small protein molecules with specific physiological activity. The efficient separation and purification of bioactive peptides contributes to the full exertion of bioactive peptides and research on their structure-activity relationship. However, the separation process is difficult and tedious. In view of this, researchers have developed mixed-mode chromatography (MMC) to separate and purify bioactive peptides by virtue of the differences in their properties such as hydrophilic, hydrophobic and charge characteristics, which exerts multiple retention mechanisms through the multifunctional stationary phases and the adjustable mobile phases. MMC has the advantages of controllable stationary phase, specific recognition and high efficiency. The development of MMC stationary phases and their application in the separation of bioactive peptides are summarized in this paper in order to provide a reference for the efficient utilization of bioactive peptides.

Keywords

• mixed-mode chromatography (mmc); chromatographic stationary phase; bioactive peptide; separation