Inhibiting effect of immunoeffector cells induced by denderitic cells vaccine on growth of PC3 and BEL7402

Abstract

Objective: To compare effect of immunoprevention or immunotherapy based on denderitic cells (DCs), or supernatants on pancreatic carcinoma and hepatocelluar carcinoma in vitro and in vivo. Methods: DCs and monouclear cells (immunoeffecetor cells) were stimulated with hGM-CSF, hIL-4, hTNF-α, PC3 TA or BEL7402 TA and hIL-2, then DCs and immunoeffector cells were cocultured, and supernatants were harvested. In vitro, the immunoeffector cells were divided into A0 group (without DCs stimulated), A1 group (DCs stimulated, cultured with cytokines cocktail), A2 group (DCs stimulated, cultured with cytokines cocktail and tumor antigen, DCs vaccine). Cytoxicity assay was performed with lactate dehydogenase method. In vivo, the nude mice were allocated in 3 groups: prevention group, receiving immunoeffector cells activated by DCs vaccine 2 days before inoculation with PC3 or BEL7402; treatment group, receiving immunoeffector cells activated by DCs vaccine after development of implanted tumor in all nude mice; control group, receiving equivalents amount of RPMI1640 cultured liquid. On the 45th day, all the nude mice were sacrificed and the tumor was weighed. Results: The maximal inhibition rate of the A0, A1 and A2 were 3.5%, 68.1%, 81.0% in the BEL7402; 4.5%, 33.0%, 62.4% in the PC3. The differences in tumor weight among three groups were significant, but the differemce were not significant between the PC3 and BEL7402. Conclusions: DCs vaccine or supernatants may play an important role in treating and preventing against malignant tumor.

Keywords

• Dendritic cells
• Pancreatic carcinoma
• Hepatocelluar carcinoma
• Immunoprevention
• Immunotherapy