Hinokitiol suppressed pan-histone expression and cell growth in oral squamous cell carcinoma cells

Yin-Hua Shih; Kuo-Wei Chang; Cheng-Chia Yu; Ming-Ching Kao; Michael Yuanchien Chen; Tong-Hong Wang; Tzu-Yun Chi; Yi-Ling Chen; Tzong-Ming Shieh

Journal of Functional Foods (May 2015)

Hinokitiol suppressed pan-histone expression and cell growth in oral squamous cell carcinoma cells

Yin-Hua Shih,
Kuo-Wei Chang,
Cheng-Chia Yu,
Ming-Ching Kao,
Michael Yuanchien Chen,
Tong-Hong Wang,
Tzu-Yun Chi,
Yi-Ling Chen,
Tzong-Ming Shieh

Affiliations

Yin-Hua Shih: Institute of Oral Biology, School of Dentistry, National Yang-Ming University, No. 155, Sec. 2, Linong Street, Taipei 11217, Taiwan; Institute of Oral Science, School of Dentistry, Chung Shan Medical University, No. 110, Sec. 1, Jianguo N. Road, Taichung 40201, Taiwan
Kuo-Wei Chang: Institute of Oral Biology, School of Dentistry, National Yang-Ming University, No. 155, Sec. 2, Linong Street, Taipei 11217, Taiwan; Department of Dentistry, Taipei Veterans General Hospital, No. 201, Sec. 2, Shipai Rd., Beitou District, Taipei 11217, Taiwan
Cheng-Chia Yu: Institute of Oral Science, School of Dentistry, Chung Shan Medical University, No. 110, Sec. 1, Jianguo N. Road, Taichung 40201, Taiwan
Ming-Ching Kao: Department of Biological Science and Technology, College of Life Sciences, China Medical University, No. 91 Hsueh-Shih Road, Taichung 40402, Taiwan
Michael Yuanchien Chen: Department of Oral & Maxillofacial Surgery, China Medical University Hospital, No.2, Yude Rd., North Dist., Taichung City 40447, Taiwan; School of Dentistry, College of Medicine, China Medical University, No. 91 Hsueh-Shih Road, Taichung 40402, Taiwan
Tong-Hong Wang: Tissue Bank, Chang Gung Memorial Hospital, No.5, Fusing St., Gueishan Dist., Taoyuan City 33305, Taiwan
Tzu-Yun Chi: Department of Dental Hygiene, College of Health Care, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan
Yi-Ling Chen: Department of Dental Hygiene, College of Health Care, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan
Tzong-Ming Shieh: Department of Dental Hygiene, College of Health Care, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan; Oral Biology Laboratory, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan; Corresponding author. Department of Dental Hygiene, College of Health Care, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan. Tel.: +886 4 22053366 7707; fax: +886 4 22073556.

Journal volume & issue: Vol. 15
pp. 452 – 463

Abstract

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Hinokitiol is reported to inhibit oral squamous cell carcinoma cells growth but its mechanism of action remains unclear. Hinokitiol induced cell cycle arrest in G1 or G1/S phase and induced cell apoptosis in oral squamous cell carcinoma cells. The cDNA microarray data showed 6.25–12.5 µM hinokitiol suppressed pan-histone mRNA expression and quantitative RT-PCR and western blotting showed the reproducibility of the results. Hinokitiol impaired the chromatin folding in oral squamous cell carcinoma cells. In addition, the binding of FLICE-associated huge protein and nuclear protein of the ataxia telangiectasia mutated locus on histone H4/e promoter was decreased, and H4/e promoter activity was significantly decreased. The malignant phenotypes of oral squamous cell carcinoma cells were suppressed in low dose (0.75–6.25 µM hinokitiol) in vitro. Feeding 10 mg/kg hinokitiol to mice suppressed xenograft tumourigenicity of HSC-3 cells in vivo. Hinokitiol showed the efficacy against oral squamous cell carcinoma cells growth by pan-histone suppression.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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