Hypoglycemic effect of silychristin A from Silybum marianum fruit via protecting pancreatic islet β cells from oxidative damage and inhibiting α-glucosidase activity in vitro and in rats with type 1 diabetes

Ningbo Qin; Xu Hu; Shengge Li; Jian Wang; Zhanlin Li; Dahong Li; Fanxing Xu; Ming Gao; Huiming Hua

Journal of Functional Foods (Nov 2017)

Hypoglycemic effect of silychristin A from Silybum marianum fruit via protecting pancreatic islet β cells from oxidative damage and inhibiting α-glucosidase activity in vitro and in rats with type 1 diabetes

Ningbo Qin,
Xu Hu,
Shengge Li,
Jian Wang,
Zhanlin Li,
Dahong Li,
Fanxing Xu,
Ming Gao,
Huiming Hua

Affiliations

Ningbo Qin: Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China
Xu Hu: Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China
Shengge Li: Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China
Jian Wang: Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China
Zhanlin Li: Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China
Dahong Li: Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China; Corresponding authors at: Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
Fanxing Xu: Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China; Corresponding authors at: Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
Ming Gao: School of Pharmaceutical Sciences, Mukogawa Women’s University, Nishinomiya 663-8179, Japan
Huiming Hua: Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China; Corresponding authors at: Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.

Journal volume & issue: Vol. 38
pp. 168 – 179

Abstract

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In the present study, the hypoglycemic effect of silychristin A on streptozotocin (STZ)-induced type 1 diabetes in rats was evaluated and the underlying pharmacological mechanism was further elucidated in vitro. Silychristin A significantly lowered the glucose level, increased insulin secretion, and improved the structure of β cells in STZ-induced rats. Accordantly, reactive oxygen species (ROS) production induced by STZ or high concentration of glucose (HG) was effectively suppressed by silychristin A in pancreatic INS-1 β cells evaluated by flow cytometry. Western blot analysis demonstrated that silychristin A markedly suppressed STZ or HG induced cell apoptosis. In addition, in vitro inhibitory effect of silychristin A on α-glucosidase and molecular docking analysis revealed that silychristin A could reduce the digestion rate of carbohydrates. These findings indicate that silychristin A may be useful as a potential functional food ingredient against oxidative stress-mediated destruction of pancreatic islet β cells and inhibition of α-glucosidase activity.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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