Untargeted Metabolomics Based on Ultra-high Performance Liquid Chromatography-Mass Spectrometry for Analysis of Differential Metabolites in Lactobacillus-fermented Ultra-fine Whole Pulp of Chimonobambusa quadrangularis Shoot

Abstract

In this work, a non-targeted metabolomic study was performed on the ultrafine whole pulp of Lactobacillus-fermented Chimonobambusa quadrangularis shoot using ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS). Based on the variable importance in projection (VIP) > 1 and P < 0.05 in t-test, 361, 392 and 398 differential metabolites were selected in raw (control) versus Limosilactobacillus reuteri (LR) fermented samples, control versus Lactobacillus plantarum (LP) fermented samples, and control and LR + LP (LRP) fermented samples in the positive ion mode; and 268, 304 and 321 differential metabolites in the negative ion mode, respectively. The major differential metabolites were lipids and lipid-like compounds, organic acids and their derivatives, organic heterocyclic compounds, and benzenes including histamine, D-mannan, indole-3-lactic acid, DL-4-hydroxyphenyl lactate, ornithine, and proline. Four significant metabolic pathways were identified by Kyoto Encyclopedia of Genes and Genomes metabolic pathway enrichment analysis, namely purine metabolism, pyrimidine metabolism and phenylalanine metabolism and citric acid cycle. This study provides theoretical support for the development of fermented bamboo shoot products.

Keywords

• chimonobambusa quadrangularis shoot; untargeted metabolomics; differential metabolites; limosilactobacillus reuteri; lactobacillus plantarum