Identification of flavonoids in litchi (Litchi chinensis Sonn.) leaf and evaluation of anticancer activities

Lingrong Wen; Dan Wu; Yueming Jiang; K. Nagendra Prasad; Sen Lin; Guoxiang Jiang; Jirui He; Mouming Zhao; Wei Luo; Bao Yang

Journal of Functional Foods (Jan 2014)

Identification of flavonoids in litchi (Litchi chinensis Sonn.) leaf and evaluation of anticancer activities

Lingrong Wen,
Dan Wu,
Yueming Jiang,
K. Nagendra Prasad,
Sen Lin,
Guoxiang Jiang,
Jirui He,
Mouming Zhao,
Wei Luo,
Bao Yang

Affiliations

Lingrong Wen: Key Laboratory of Plant Resource Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China
Dan Wu: Key Laboratory of Plant Resource Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; University of Chinese Academy of Sciences, Beijing 100049, China
Yueming Jiang: Key Laboratory of Plant Resource Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
K. Nagendra Prasad: School of Engineering, Monash University, Selangor 46150, Malaysia
Sen Lin: Key Laboratory of Plant Resource Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
Guoxiang Jiang: Key Laboratory of Plant Resource Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
Jirui He: Key Laboratory of Plant Resource Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; University of Chinese Academy of Sciences, Beijing 100049, China
Mouming Zhao: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China
Wei Luo: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China
Bao Yang: Key Laboratory of Plant Resource Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; Corresponding author. Tel.: +86 20 37083042; fax: +86 20 37252960.

Journal volume & issue: Vol. 6
pp. 555 – 563

Abstract

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Six flavonoids, namely luteolin (1), epicatechin (2), kaempferol 3-O-β-glucoside (3), kaempferol 3-O-α-rhamnoside (4), procyanidin A2 (5) and rutin (6) were purified from the EtOAc-soluble extract of litchi leaf by column chromatography. Their structures were elucidated by nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS) evidences. Luteolin and kaempferol 3-O-α-rhamnoside were found from this plant for the first time. Procyanidin A2 exhibited high anticancer activities against human hepatoma HepG2 and human cervical carcinoma Hela cells. However, it had poor anticancer activities against human lung cancer A549 and human breast cancer MCF-7 cells. Luteolin, epicatechin, procyanidin A2 and rutin showed good antioxidant activities than butylated hydroxytoluene (BHT). The antimicrobial activity assay indicated that luteolin possessed the strongest antimicrobial activity against Staphylococcus aureus, Escherichia coli, Shigella dysenteriae, Salmonella and Bacillus thuringiensis. Epicatechin, procyanidin A2 and rutin showed relatively weak antimicrobial activities.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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